Journal: Cancer Imaging

Article Title: Characteristics of 18 F-FAPI-04 PET/CT in patients with peritoneal metastasis and to predict treatment efficacy, a head-to-head comparison with 18 F-FDG PET/CT

doi: 10.1186/s40644-025-00887-9

Figure Lengend Snippet: Comparison of 18 F-FAPI-04 Uptake Parameters Across FAP-IHC and α-SMA-IHC Score Groups. Bar plots demonstrate significant differences in 18 F-FAPI-04 uptake parameters among groups with FAP-IHC scores 1, 2, and 3 ( A-C ), A : SUV max ; B : T/B; C : T/L. Bar plots show no statistically significant differences in 18 F-FAPI-04 uptake parameters across α-SMA-IHC score groups 1, 2, and 3( D-F ), A : SUV max ; B : T/B; C : T/L

Article Snippet: The expression of FAP and α-SMA in cancer tissues was examined by immunohistochemistry (IHC) using FAP antibody (BOSTER Biological Technology Co., Ltd) or α-SMA antibody (Servicebio Technology Co., Ltd, GB13044), both diluted at 1:200.

Techniques: Comparison

Journal: Cancer Imaging

Article Title: Characteristics of 18 F-FAPI-04 PET/CT in patients with peritoneal metastasis and to predict treatment efficacy, a head-to-head comparison with 18 F-FDG PET/CT

doi: 10.1186/s40644-025-00887-9

Figure Lengend Snippet: 18 F-FAPI PET/CT imaging and FAP immunohistochemical results (×400, DAB staining) in peritoneal metastases of pancreatic and gastric cancers. A : 18 F-FAPI-04 PET/CT of gastric cancer, B : α-SMA expression in gastric cancer, C : FAP expression in gastric cancer, D : 18 F-FAPI-04 PET/CT of pancreatic cancer, E : α-SMA expression in pancreatic cancer, F : FAP expression in pancreatic cancer. FAP expression in pancreatic cancer was higher than that in gastric cancer

Article Snippet: The expression of FAP and α-SMA in cancer tissues was examined by immunohistochemistry (IHC) using FAP antibody (BOSTER Biological Technology Co., Ltd) or α-SMA antibody (Servicebio Technology Co., Ltd, GB13044), both diluted at 1:200.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Immunohistochemical staining, Staining, Expressing

Journal: Cancers

Article Title: NAB 2-Expressing Cancer-Associated Fibroblast Promotes HNSCC Progression

doi: 10.3390/cancers11030388

Figure Lengend Snippet: CAF marker and matrix metalloproteinase (MMP) expression in primary fibroblasts from HNSCC patient tissue. ( A ) CAFs and paired non-tumor fibroblasts (NTFs) were isolated from three HNSCC patient tissue (P1, P2, P3) specimens by short-term primary culture. α-SMA and FAP mRNA and protein levels were evaluated by real-time quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. ( B ) MMP mRNA and protein expression was analyzed under the same conditions. Results represent the mean ± standard deviation of two or three experiments.

Article Snippet: Rabbit anti-fibroblast activation protein (FAP) antibody was from GeneTex (GeneTex, Inc., Irvine, CA, USA).

Techniques: Marker, Expressing, Isolation, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal:

Article Title: High frequency of autoantibodies in patients with primary sclerosing cholangitis that bind biliary epithelial cells and induce expression of CD44 and production of interleukin 6

doi:

Figure Lengend Snippet: Isolated biliary epithelial cells stained positive for cytokeratin 7 (A), cytokeratin 19 (B), and a common cytokeratin epitope (C). Flow cytometric analysis indicated that the same cells stained negative for von Williebrand factor (dotted curve) (endothelial cells), smooth muscle (dashed curve), and antifibroblast antibodies (light grey curve). A negative control using only secondary antibodies was also included (black curve) (D).

Article Snippet: Antibodies against surface receptors included non-conjugated antifibroblast antibodies (Harlan Sera-Lab) and FITC conjugated antibodies to von Willebrand factor (Serotec, UK).

Techniques: Isolation, Staining, Negative Control